Methods and Techniques of Biochemical Analysis I

Abstract

This module, titled "Biochemical Analysis Methods and Techniques I," constitutes the methodological cornerstone of your Biochemistry Bachelor's degree. Offered within the Methodological Teaching Unit (UEM1), it carries 5 credits and a coefficient of 3, underscoring its fundamental importance in the curriculum of a Bachelor’s degree in Biochemistry. The central aim of this course is to equip the student with a complete and essential toolkit for tackling any analytical challenge in the life sciences. This module is dedicated to acquiring practical and theoretical skills. The module explore two major pillars: - Sample preparation and fractionation methods (centrifugation, dialysis, lyophilization...), which are the indispensable steps for obtaining clean, concentrated biological material prior to any analysis. - The fundamental principles of spectroscopy (UV-Visible, Infrared, Atomic), which form the physical basis for most modern detection and quantification instruments. By mastering these techniques, the student will be able to develop a critical analytical mindset and will learn to choose the most suitable method for a given problem, understand its limitations, and correctly interpret the results it provides. This course builds upon the knowledge acquired in Biology, General Biochemistry, and Chemistry. It, in turn, serves as an indispensable foundation for practical laboratory work, final-year projects, and future professional life. We wish to each student a stimulating exploration into the heart of the instruments and principles that make the invisible visible and discover the molecular language of life.